Product Name:
Mouse Macrophage Stimulating Protein (MSP) ELISA Kit
Cat.#:
JL49928
Regulatory Status:
Alternative:
MST1; HGFL; NF15S2; Hepatocyte Growth Factor-Like Protein; Macrophage Stimulating 1
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.15-10ng/mL
Sensitivity:
0.06ng/mL
Specificity:
This kit recognizes Mouse MSP in samples. No significant cross-reactivity or interference between Mouse MSP and analogues was observed.
Sample Type:
Serum, plasma and other biological fluids.
Assay Procedure:

Research Area:
Cancer
Product Datasheets
| MSP
Macrophage-stimulating protein (MSP), also known as hepatocyte growth factor-like protein (HLP, HGFL, or HGFLP), is a protein that is encoded by the MST1 (macrophage-stimulating 1) gene.| Typical data
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.Concentration (ng/mL) | 10 | 5 | 2.5 | 1.25 | 0.62 | 0.31 | 0.15 | 0 |
OD | 2.04 | 1.61 | 1.23 | 0.84 | 0.53 | 0.35 | 0.23 | 0.08 |
Corrected OD | 1.96 | 1.53 | 1.15 | 0.76 | 0.45 | 0.27 | 0.15 | - |
| Repeatability
Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%| Recovery
The recovery of Mouse MSP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.Sample Type | Range(%) | Average Recovery(%) |
Serum(n=8) | 84-102 | 96 |
Plasma(n=8) | 92-105 | 101 |
| Linearity
Samples were spiked with high concentrations of Mouse MSP and diluted with Universal Diluent to produce samples with values within the range of the assay.Serum(n=4) | Plasma(n=4) | ||
1 : 2 | Range (%) | 84-95 | 88-96 |
Average (%) | 91 | 93 | |
1 : 4 | Range (%) | 89-103 | 87-108 |
Average (%) | 94 | 98 |






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